Journal: Journal of Inflammation (London, England)

Article Title: Exosomes derived from M2 macrophages regulate airway inflammation by modulating epithelial cell proliferation and apoptosis

doi: 10.1186/s12950-025-00444-y

Figure Lengend Snippet: Identification of M2Φ and M2Φ-Exosomes. A Morphological analysis of M2Φ under a microscope. Scale bar = 100 nm. B - G Gene expression of M2Φ-associated markers (Arg1, Mrc1, and YM1) and M1Φ-associated markers (TNF-α, IL-1β and CD86) quantified by RT-qPCR. H Expression of surface biomarkers F4/80 and CD206 on M2Φ, determined by flow cytometry. I Extraction process of M2Φ-derived exosomes (M2Φ-Exos). J Transmission electron microscopy (TEM) of exosomes isolated from the supernatant of M2Φ. Scale bar = 100 nm. K Size distribution of M2Φ-Exos measured by nanoparticle tracking analysis (NTA). L Western blot analysis of exosome marker proteins CD9, TSG101, and CD63, along with the endoplasmic reticulum marker protein Calnexin. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: After blocking with a fast blocking solution (Beyotime, Beijing, China) for 20 min at room temperature, the membranes were incubated overnight at 4 °C with primary antibodies: rabbit anti-mouse CD9 (1:1000, Zen Bioscience, Chengdu, China), CD63 (1:1000, Zen Bioscience, Chengdu, China), TSG101 (1:1000, Zen Bioscience, Chengdu, China), Calnexin (1:1000, Zen Bioscience, Chengdu, China), Bcl2 (1:500, Zen Bioscience, Chengdu, China), Bax (1:1000, Zen Bioscience, Chengdu, China), Caspase 3 (1:2000, Proteintech, Wuhan, China), Cleaved-caspase 3 (1:1000, Proteintech, Wuhan, China), PCNA (1:3000, Proteintech, Wuhan, China), and β-actin (1:10000, Zen Bioscience, Chengdu, China).

Techniques: Microscopy, Gene Expression, Quantitative RT-PCR, Expressing, Flow Cytometry, Extraction, Derivative Assay, Transmission Assay, Electron Microscopy, Isolation, Western Blot, Marker